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Azura 2X HS Taq Red Mix 1000 Reactions / 20 x 1.25 mL
94% of respondents would recommend this to a friend
USD 807
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Azura 2x HS Taq Red Mix - Market-leading specificity with a wide range of DNA templates
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What Stands Out
Product Details
- Azura 2x HS Taq Red Mix is a ready-to-use master mix optimized for high specificity with a wide range of DNA templates including difficult GC-rich targets.
- Formulated with Azura HS Taq polymerase, hot-start antibodies, a proprietary PCR enhancer, and reaction buffer containing optimal levels of dNTPs and MgCl2.
- Allows for fast cycling conditions with improved efficiency and reproducibility.
- Contains an inert red dye and high-density additive, allowing samples to be loaded directly in agarose gels without additional gel loading or tracking dyes.
- The red dye migrates at a rate of 600 bp and 350 bp DNA in 1% and 2% TAE agarose gels, respectively.
- Suitable for applications such as multiplex PCR, fast PCR, genotyping, colony PCR, and low-copy PCR.
| Item Form | Gel |
| Volume | 20 ml |
| Brand Name | Azura Genomics |
| Manufacturer | Azura Genomics |
| Part Number | AZ-1625 |
| Brand | Azura Genomics |
| Item Volume | 20 ml |
| Item Weight | 1 lbs (450 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists requiring high efficiency and sensitivity in PCR amplification for various applications.
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Research Labs
Perfect for research laboratories conducting large-scale experiments with its capacity for 1000 reactions.
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Educational Institutions
Useful for schools and universities where a reliable, cost-effective PCR solution is necessary for student experiments.
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Beginner Users
Less suitable for those unfamiliar with PCR protocols as it may require specific handling and optimization.
Product Description
Azura 2X HS Taq Red Mix 1000 Reactions / 20 x 1.25 mL
Customer Questions & Answers
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Question:
What is Azura 2X HS Taq Red Mix and how is it used?
Answer: Azura 2X HS Taq Red Mix is a high-performance polymerase mixture designed for multiplex PCR, qPCR, and various other amplification methods. It contains premium quality Taq DNA polymerase along with buffer and additives that enhance specificity and yield. This makes it incredibly efficient for researchers needing reliable and reproducible results in gene amplification. It's perfect for applications like cloning, sequencing, and diagnostics, providing researchers with consistent performance even in challenging conditions. -
Question:
How do I prepare a PCR reaction using Azura 2X HS Taq Red Mix?
Answer: To prepare a PCR reaction using Azura 2X HS Taq Red Mix, mix the required volume of the mix with your specific primers, template DNA, and any additional reagents as needed. Because the mix is 2X concentrated, remember to dilute it appropriately to ensure optimal reaction volume. By following the manufacturer's protocol and adjusting for your specific requirements, you can achieve consistent results, particularly in complex or high-throughput assays where precision is crucial. -
Question:
What types of samples can I use with Azura 2X HS Taq Red Mix?
Answer: Azura 2X HS Taq Red Mix is versatile and can be used with a variety of DNA templates, including genomic DNA, plasmid DNA, and cDNA. It is particularly effective with samples that may be difficult to amplify, ensuring that even low-abundance targets can be successfully amplified. This versatility makes it ideal for many applications in molecular biology, such as gene expression studies and genetic fingerprinting, where different sample types might be encountered. -
Question:
What are the storage conditions for Azura 2X HS Taq Red Mix?
Answer: Azura 2X HS Taq Red Mix should be stored at -20°C for long-term preservation. It is advisable to avoid repeated freeze-thaw cycles, which can affect the enzyme's performance. For convenience, aliquoting the mix into smaller tubes can help prevent repeated freeze-thaw cycles. By properly storing the mix, users can maintain the integrity and efficiency of the enzyme for consistent, high-quality PCR results. -
Question:
Can Azura 2X HS Taq Red Mix be used for quantitative PCR?
Answer: Yes, Azura 2X HS Taq Red Mix is suitable for quantitative real-time PCR (qPCR) applications. Its formulation is optimized to provide high specificity and sensitivity, making it an excellent choice for quantification of nucleic acids in samples. Researchers can achieve reliable threshold cycle (Ct) values necessary for quantitative analysis, ensuring robust performance in experiments aimed at gene expression analysis and pathogen detection. -
Question:
What is the significance of using a red dye in Azura 2X HS Taq Red Mix?
Answer: The inclusion of a red dye in Azura 2X HS Taq Red Mix serves multiple purposes. It enables visual tracking of the reaction progress during electrophoresis, and it helps to minimize the risk of pipetting errors by providing a colorimetric reference. This feature is particularly useful in educational settings or labs where multiple samples are processed simultaneously, as it streamlines workflows and enhances accuracy in detecting and analyzing PCR products. -
Question:
Is Azura 2X HS Taq Red Mix applicable for high-throughput applications?
Answer: Absolutely! Azura 2X HS Taq Red Mix is designed to meet the demands of high-throughput applications often encountered in genomic laboratories. The robust performance and reliability of this mix allow researchers to efficiently run numerous samples simultaneously without compromising on quality. This adaptability makes it essential for projects such as genome-wide association studies and large-scale screening, where efficiency and speed are paramount. -
Question:
What is the buffer composition of Azura 2X HS Taq Red Mix?
Answer: Azura 2X HS Taq Red Mix contains a specially formulated buffer system that optimizes enzyme activity and ensures robust performance across a wide variety of templates. This buffer consistently provides the necessary pH and ionic strength to support Taq polymerase during the amplification process. Understanding the buffer composition helps researchers tailor their PCR conditions to their specific amplification goals, achieving optimal results with diverse target sequences. -
Question:
Can I modify the PCR conditions while using Azura 2X HS Taq Red Mix?
Answer: Yes, while it’s important to follow the recommended protocols initially, modifications to PCR conditions like annealing temperature, extension time, and cycle number can be made based on your target's complexity and the assay design. Fine-tuning these parameters can enhance specificity and yield, tailoring the reaction to meet specific research needs, particularly when working with challenging DNA targets or complex templates. -
Question:
Where can I buy Azura 2X HS Taq Red Mix 1000 Reactions / 20 X 1.25 ML?
Answer: You can purchase Azura 2X HS Taq Red Mix 1000 Reactions / 20 X 1.25 ML from Ubuy. They offer a convenient online shopping experience for laboratory products in Turks and Caicos, making it easy to acquire this essential tool for your molecular biology work.
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USD 807
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Features & Benefits
- Ready-to-use master mix optimized for specificity
- Suitable for difficult GC-rich targets
- High-performance formulation with Azura HS Taq polymerase
- Allows fast cycling conditions with efficiency and reproducibility
- Contains inert red dye for direct gel loading
- Compatible with multiplex PCR, fast PCR, genotyping, colony PCR, and low-copy PCR
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