Bradford Protein Assay w/BSA Protein Standard, 500 Assays, #10478- by Cepham Life Sciences
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Bradford Protein Assay w/BSA Protein Standard, 500 Assays, #10478- by Cepham Life Sciences
Bradford Protein Assay w/BSA Protein Standard, 500 Assays, #10478- by Cepham Life Sciences
Item #: 32516304

Bradford Protein Assay w/BSA Protein Standard, 500 Assays, #10478- by Cepham Life Sciences

Item #: 32516304

USD 148

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What Stands Out

Comprehensive Kit
Includes a BSA protein standard for accurate quantification, making it ideal for diverse laboratory applications and ensuring consistent, reliable results across 500 assays.
High Sensitivity
Designed for precise measurement with enhanced sensitivity, helping researchers detect low protein concentrations effectively, surpassing competitor assays in performance.
Easy Protocol
User-friendly methodology simplifies the protein quantitation process, reducing complexity in workflows and allowing for efficient implementation in various research settings.

Product Details

Shop Bradford Protein Assay w/BSA Protein Standard, 500 Assays, #10478- by Cepham Life Sciences online at a best price in Turks and Caicos. B07DPSNCPF
Date First Available June 13, 2018
Manufacturer Cepham Life Sciences
Brand NameCepham Life Sciences
Part Number10478
Item Weight1 lbs (450 grams)

Who Should Buy?

Suitable For
  • Laboratory Researchers

    Ideal for researchers needing quick, reliable protein concentration measurements in various biological samples.

  • Biotechnology Companies

    A valuable tool for biotech firms conducting protein quantification in product development and quality assurance.

  • Educational Institutions

    Excellent for teaching labs, accommodating hands-on learning in biochemistry and molecular biology courses.

Not Suitable For
  • Non-Scientific Users

    Individuals without a scientific background may find usage and interpretation of the assay challenging.

Product Description

Bradford Protein Assay w/BSA Protein Standard, 500 Assays, #10478- by Cepham Life Sciences

About This Item

Introducing the Bradford Protein Assay wBSA Protein Standard by Cepham Life Sciences. This highly optimized and ready-to-use protein assay is designed for fast and accurate quantitation of total protein. The assay uses the Coomassie Brilliant Blue (CBB) G-250 dye, which binds to proteins and undergoes a color change from brown to blue.

By measuring the absorbance at 595nm using a spectrophotometer or microplate reader, you can quickly determine the protein concentration in your sample. The Bradford Protein Assay is known for its reliability and accuracy. It specifically binds to basic (arginine) and aromatic amino acid residues in proteins, making it suitable for a wide range of samples. The assay's constant extinction coefficient ensures precise quantitation over a 10-fold concentration range. For your convenience, we provide a BSA Protein Standard with the kit.

Bovine serum albumin (BSA) is used as the standard because it produces a greater color development in the Bradford assay compared to other proteins. By including this standard, you can easily calibrate your results and ensure accuracy. Whether you prefer to use a cuvette or microplate format, the Bradford Protein Assay is easy to perform. Simply combine a small amount of your protein sample with the assay reagent, mix, and incubate briefly.

Then, measure the absorbance at 595nm and obtain your protein concentration. With each kit, you'll have enough reagents to perform 500 standard test tube assays or 2,500 standard micro-well assays. This allows for multiple measurements and ensures you can accurately quantify protein levels in your samples. Trust Cepham Life Sciences to deliver high-quality protein assays for your research needs. The Bradford Protein Assay wBSA Protein Standard is an essential tool for any scientist or researcher looking to accurately quantify protein concentrations.

Order yours today and experience the convenience and reliability of this advanced protein assay.

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Customer Questions & Answers

  • Question: What is the Bradford Protein Assay wBSA Protein Standard used for?

    Answer: The Bradford Protein Assay wBSA Protein Standard is primarily used for quantifying protein concentrations in various biological samples. This assay relies on the binding of the dye Coomassie Brilliant Blue G-250 to proteins, which results in a color change that can be measured spectrophotometrically. This method is widely used in laboratories for applications such as protein purification, enzyme activity studies, and studying protein interactions, making it essential for researchers in biochemistry, molecular biology, and related fields.
  • Question: How many assays are included in the Bradford Protein Assay Kit?

    Answer: The Bradford Protein Assay Kit includes enough reagents for performing 500 assays. This makes it an economical choice for laboratories that conduct frequent protein concentration analyses. With this high assay count, researchers can efficiently complete multiple experiments without the need to frequently restock, suitable for high-throughput screening or routine quality control in protein studies.
  • Question: What are the advantages of using the Bradford Protein Assay?

    Answer: One of the primary advantages of the Bradford Protein Assay is its simplicity and speed, allowing for rapid determination of protein concentrations in under an hour. Additionally, the assay is compatible with various buffers and does not require extensive sample preparation, making it accessible for all laboratory setups. Researchers often prefer this method for its high sensitivity, which can detect protein concentrations as low as 1 µg/mL, making it ideal for both pure proteins and complex biological samples.
  • Question: Is the Bradford Assay method suitable for all protein types?

    Answer: While the Bradford Assay is widely used, it is important to note that it may not be suitable for all types of proteins, particularly those that do not bind well to Coomassie dye or those that have a high content of detergents or strong reducing agents. Proteins like certain glycoproteins and those with low aromatic amino acid content might yield less accurate results. For these cases, alternative assays such as the BCA or Lowe assays may be recommended.
  • Question: How does the Bradford Assay compare to other protein quantification methods?

    Answer: The Bradford Assay is often favored for its rapidity and ease of use when compared to other methods such as BCA or UV absorbance. While alternative methods may offer better specificity for certain protein types, the Bradford Assay delivers reliable results for a broad range of samples and is particularly effective for routine applications. Researchers might choose it for its cost-effectiveness and minimal sample volume requirements in comparison to other more complex assays.
  • Question: Can the Bradford Protein Assay be used for samples containing detergents?

    Answer: The Bradford Protein Assay can be affected by the presence of detergents, as they can interfere with the dye-protein interaction and lead to inaccurate readings. However, non-ionic detergents at low concentrations are generally acceptable. It’s best for researchers to evaluate their specific samples and possibly conduct parallel tests using another quantification method before employing the Bradford Assay in a detergent-rich environment.
  • Question: What is the optimal temperature for conducting the Bradford Protein Assay?

    Answer: The optimal temperature for performing the Bradford Protein Assay is room temperature, typically around 20-25°C. This allows for the best dye-protein interactions and color development, aiding accurate spectrophotometric measurements. Performing the assay at consistent temperatures within this range helps to ensure reproducibility of results, which is crucial for any quantitative analysis.
  • Question: Can I store the Bradford Protein Assay reagents for later use?

    Answer: Yes, the Bradford Protein Assay reagents can be stored for later use. Typically, the dye solution can be stable for several months when protected from light and kept at appropriate temperatures as specified by the manufacturer. Storing the reagents allows researchers to prepare only the necessary amounts needed for specific experiments while minimizing waste, making it practical for laboratories with varying assay demands.
  • Question: Who can benefit from using the Bradford Protein Assay?

    Answer: Researchers, students, and professionals in fields such as biochemistry, molecular biology, and clinical diagnostics can greatly benefit from using the Bradford Protein Assay. Its user-friendly nature and ability to provide quick and reliable protein quantification make it a preferred choice for routine laboratory work, including research institutions and educational settings, where hands-on experience with protein analysis is essential.
  • Question: Where can I buy Bradford Protein Assay wBSA Protein Standard, 500 Assays, 10478- by Cepham Life Sciences?

    Answer: You can purchase the Bradford Protein Assay wBSA Protein Standard, 500 Assays, 10478- by Cepham Life Sciences through Ubuy in Turks and Caicos. Ubuy offers a convenient platform for obtaining various scientific products, ensuring that you have access to this essential reagent for your protein analysis needs.

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Pros

  • High sensitivity and accuracy
  • Reliable results for protein quantification
  • User-friendly protocol
  • Versatile for various samples
  • Excellent customer support

Cons

  • Requires careful handling of reagents.

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