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Item #:
145470486
AzuraQuant Probe 1-Step qPCR Mix LoRox 100 Reactions / 1 mL
Item #:
145470486
USD 186
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The AzuraQuant Probe 1-Step qPCR Mix loRox delivers earlier quantification cycle values and unmatched performance in multiplexing.
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What Stands Out
Rapid PCR Performance
Designed for quick and efficient amplification, the 1-Step qPCR Mix significantly reduces reaction times while maintaining high sensitivity and specificity, ensuring reliable results for researchers.
LOROX Technology
Incorporating advanced LoRox technology, this mix offers enhanced signal detection, improving the accuracy and reproducibility of quantitative assays compared to traditional methods.
Versatile Applications
Suitable for various real-time PCR applications, this mix simplifies protocols by enabling one-step reactions, catering to diverse laboratory needs and streamlining workflows for maximum efficiency.
Product Details
| Item Weight | 0.25 lbs (110 grams) |
Who Should Buy?
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Academic Researchers
Ideal for researchers needing reliable qPCR results for academic studies and publications involving gene expression analysis.
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Diagnostics Labs
Suitable for laboratories focusing on clinical diagnostics that require efficient and accurate qPCR implementation.
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Environmental Testing
Benefits environmental scientists conducting DNA quantification on samples from soil, water, or air pollutants.
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New Users
Less suitable for those unfamiliar with qPCR, as it requires proper technique and sample preparation for optimal results.
Product Description
AzuraQuant Probe 1-Step qPCR Mix LoRox 100 Reactions / 1 mL
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Customer Questions & Answers
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Question:
What is AzuraQuant Probe 1-Step qPCR Mix LoRox used for?
Answer: AzuraQuant Probe 1-Step qPCR Mix LoRox is designed for quantitative PCR applications, allowing researchers to amplify and quantify DNA in a single step. This mix contains high-quality enzymes, buffers, and LoRox dye, which helps in real-time monitoring of the PCR process. It's ideal for applications such as gene expression analysis, pathogen detection, and genotyping. Researchers benefit from its convenience and efficiency, as it significantly reduces handling time without compromising on performance. -
Question:
How do I prepare the AzuraQuant Probe 1-Step qPCR Mix for my experiments?
Answer: To prepare the AzuraQuant Probe 1-Step qPCR Mix, simply add the required amount to your PCR reaction mixture according to your experimental design. The protocol typically includes mixing the mix with your target DNA, primers, and water. Specific instructions may vary, so it is essential to refer to the product manual for precise ratios to achieve optimal results. Using this mix ensures consistent assay performance across various experimental conditions. -
Question:
What are the storage conditions for AzuraQuant Probe 1-Step qPCR Mix?
Answer: Proper storage of AzuraQuant Probe 1-Step qPCR Mix is crucial for maintaining its functionality. It should be kept at -20°C and protected from light to prevent degradation of its components. Once the mix is thawed for use, it can be aliquoted to avoid repeated freeze-thaw cycles, which can affect its performance. Adhering to these storage conditions will help ensure the integrity of your experiments and reliable results. -
Question:
Is the AzuraQuant Probe 1-Step qPCR Mix compatible with all types of qPCR machines?
Answer: Yes, the AzuraQuant Probe 1-Step qPCR Mix is designed to be compatible with most qPCR machines on the market. This universality makes it a versatile choice for researchers employing various technologies. However, for optimal results, it is advisable to consult the user manual of your specific qPCR device and adjust thermal cycling conditions as necessary to suit the mix's performance profiles. -
Question:
What specific features make the LoRox dye advantageous?
Answer: LoRox dye included in the AzuraQuant Probe 1-Step qPCR Mix enhances signal sensitivity and increases the accuracy of quantification during the qPCR process. Its unique properties allow for more precise detection of DNA amplification, especially at low target concentrations. This is particularly beneficial in applications like rare pathogen detection or low-abundance gene expression studies, where traditional dyes may not perform as effectively. -
Question:
Can I use AzuraQuant Probe 1-Step qPCR Mix for multiplex assays?
Answer: Yes, AzuraQuant Probe 1-Step qPCR Mix is suitable for multiplex assays, enabling the simultaneous quantification of multiple targets in a single reaction tube. This capability conserves valuable samples and reduces overall assay time. When designing multiplex experiments, it’s critical to ensure that the selected probes do not interfere with each other and are spectrally distinct for accurate data analysis. -
Question:
What is the recommended number of reactions per tube for the AzuraQuant Probe 1-Step qPCR Mix?
Answer: The AzuraQuant Probe 1-Step qPCR Mix comes in a 1 mL tube, providing sufficient material for up to 100 reactions, assuming the standard volume per reaction is utilized. This concentration makes it a cost-effective choice for high-throughput applications. Researchers can efficiently conduct multiple assays without the need for repeated purchases, ensuring laboratory resources are optimally utilized. -
Question:
Are there any specific application areas for which the AzuraQuant Probe 1-Step qPCR Mix is particularly recommended?
Answer: The AzuraQuant Probe 1-Step qPCR Mix is highly recommended for a variety of applications, including gene expression studies, microbial quantification, and cloning validations. Its robustness in different PCR setups ensures reliable results across diverse research needs. Laboratories focusing on gene-related research or clinical diagnostics can particularly benefit from its high-performance capabilities. -
Question:
How does AzuraQuant Probe 1-Step qPCR Mix compare to traditional two-step qPCR methods?
Answer: The AzuraQuant Probe 1-Step qPCR Mix streamlines workflows by combining the reverse transcription and amplification steps into a single step, unlike traditional two-step methods that separate these processes. This reduction in handling time not only enhances throughput but also minimizes the risk of contamination. Users can expect improved efficiency, especially in high-demand settings requiring rapid results. -
Question:
Where can I buy AzuraQuant Probe 1-Step qPCR Mix LoRox 100 Reactions / 1 mL in Turks and Caicos?
Answer: You can purchase the AzuraQuant Probe 1-Step qPCR Mix LoRox 100 Reactions / 1 mL from Ubuy in Turks and Caicos. Ubuy offers a wide selection of laboratory supplies, including various specialized reagents and kits, ensuring you have access to the necessary tools for your research needs.
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USD 186
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Features & Benefits
- Ready-to-use 2x master mix for sensitive RT-qPCR.
- Compatible with various probe-detection technologies including TaqMan and Scorpions.
- Offers robust cDNA synthesis and real-time PCR in a single tube.
- Delivers earlier quantification cycle values (Ct) for increased sensitivity.
- Requires little optimization, reducing pipetting steps and time to results.
- Superior multiplex capability for detecting low-copy numbers and various gene expressions.
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